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To study whether RAI recruitment of p85 was indeed mediated by GAB1, we first investigated whether RAI, GAB1 and p85 were able to form stable complexes in vivo. To this aim, we used 293 cells transiently transfected with different combinations of cDNAs encoding RAI, GAB1 and oncogenic RET/MEN2A. In a first set of experiments, we showed a complex between RAI and GAB1 by reciprocal coimmunoprecipitation and Western blotting (Figure ugg bailey button 1a). Carr highly critical of the Internet’s effect on cognition. It was published in the July/August 2008 edition of The Atlantic magazine as a six page cover story.[1] The essay builds upon Carr’s book The Big Switch: Rewiring the World, From Edison to Google, in particular the last chapter, “iGod”.[2] Carr’s main argument is that the Internet might have detrimental effects on cognition that diminish the capacity for concentration and contemplation. As the title indicates, the article specifically targets Google, although it also generalizes about the cognitive impact of the whole Internet and World Wide Web.[3][4][5].